Plant Tissue Culture Ppt Pdf -

Plant Tissue Culture: Comprehensive Write-up

D. Applications

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Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues, or organs under sterile conditions on a nutrient culture medium of known composition. It relies on the concept of , which is the ability of a single plant cell to regenerate into a whole, new plant. Core Concepts and Methodology Plant tissue culture | PPTX - Slideshare

• USDA (USA) and ICAR (India) publish free, public domain PDFs on PTC for crop improvement.

1. Select healthy source plant.
2. Trim explant to desired size (0.5–1.0 cm usually).
3. Wash with running water and mild detergent (10–20 min).
4. Surface-sterilize: 70% ethanol (30–60 s) → sodium hypochlorite 0.5–2.5% available chlorine (5–15 min) with surfactant → rinse 3–5 times with sterile distilled water.
5. Transfer to sterile Petri dish under laminar flow; excise final segments with sterile tools.